Gene sequence of neuropeptide CCHamide as well as preparation method and application of gene sequence

The invention belongs to the technical field of gene engineering, and particularly relates to a gene sequence of neuropeptide CCHamide as well as a preparation method and application of the gene sequence. The nucleotide sequence of the neuropeptide CCHamide gene is as shown in SEQ ID NO. 1 (sequence...

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Bibliographische Detailangaben
Hauptverfasser: ZHANG MIN, XIA GUOXU, WANG JIALE, DING TIANBO, CHU DONG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the technical field of gene engineering, and particularly relates to a gene sequence of neuropeptide CCHamide as well as a preparation method and application of the gene sequence. The nucleotide sequence of the neuropeptide CCHamide gene is as shown in SEQ ID NO. 1 (sequence identifier number 1). The invention also provides dsRNA of the neuropeptide CCHamide gene, and the nucleotide sequence of the dsRNA is as shown in SEQ ID NO. 2. According to the invention, the transient expression vector of the bemisia tabaci MED cryptic species neuropeptide CCHamide gene is constructed to transform tobacco, and the BtCCH2 protein is over-expressed in the tobacco, so that the capability of bemisia tabaci in acquiring and spreading the tomato chlorosis virus can be remarkably reduced. The bemisia tabaci neuropeptide CCHamide gene plays an obvious role in inhibiting bemisia tabaci from acquiring and spreading the tomato chlorosis virus, and the gene plays an important role in intercepting, preventin