Bacillus licheniformis recombinant strain simultaneously utilizing glucose and mannitol

The invention discloses a bacillus licheniformis recombinant strain simultaneously utilizing glucose and mannitol, and belongs to the technical field of synthetic biology and metabolic engineering. According to the invention, mtlA, mtlF and mtlD are over-expressed in the ccpA-deleted strain, so that the metabolic pathway of mannitol is not inhibited by catabolism of glucose any more, and bacillus licheniformis can be co-utilized when facing glucose and mannitol. According to the technical scheme, the carbon source preference of bacillus licheniformis can be obviously changed, and joint utilization of glucose and mannitol can be achieved. 本发明公开了一种同时利用葡萄糖与甘露醇的地衣芽孢杆菌重组菌株，属于合成生物学与代谢工程技术领域。本发明通过在ccpA缺失菌株中过表达mtlA、mtlF和mtlD，使甘露醇代谢途径不再受到葡萄糖的分解代谢抑制，使得地衣芽孢杆菌在面对葡萄糖与甘露醇时可以共利用。本发明的技术方案可以明显改变地衣芽孢杆菌的碳源偏好性，可以实现葡萄糖与甘露醇的共同利用。