Method for detecting activity of anti-CD22 monoclonal antibody and application thereof

The invention discloses a method for detecting the activity of an anti-CD22 monoclonal antibody, which comprises the following steps: co-incubating a to-be-detected anti-CD22 monoclonal antibody and RAMOS cells, detecting the concentration of the endocytosis to-be-detected anti-CD22 monoclonal antibody, comparing the concentration of the endocytosis to-be-detected anti-CD22 monoclonal antibody with the original concentration of the to-be-detected anti-CD22 monoclonal antibody, and determining the activity of the to-be-detected anti-CD22 monoclonal antibody. The activity of the anti-CD22 monoclonal antibody to be detected is obtained. Aiming at the unique action mechanism of the anti-CD22 antibody, the invention provides a specific cell-based activity detection method which is suitable for quality control of anti-CD22 monoclonal antibodies such as SM03, SM06 and the like; the activity detection of the anti-CD22 monoclonal antibody is realized by measuring the internalization rate of the antibody combined with