Direct amplification reagent, kit and method for nucleic acid mass spectrometry detection of various human cardiovascular and cerebrovascular disease susceptibility genes

The invention provides a direct amplification reagent for nucleic acid mass spectrometric detection, a kit containing the direct amplification reagent and used for nucleic acid mass spectrometric detection of various human cardiovascular and cerebrovascular disease susceptible genes and a method. The direct amplification reagent comprises a nonionic surfactant, an alcohol compound, BSA and a Tris-HCl buffer solution, the direct amplification reagent not only can be compatible with blood and blood card samples to realize multiple amplification effects on the two samples and ensure amplification efficiency, but also is simple in components, and does not generate negative effects on a co-crystallization effect of the samples and a chip matrix during on-machine detection. The direct amplification reagent is further applied to preparation of a kit for nucleic acid mass spectrometry detection of various human cardiovascular and cerebrovascular disease susceptibility genes, 16 SNP loci can be detected at the same ti