High-yield tannase recombinant bacterium as well as preparation method and application thereof

The invention discloses a high-yield tannase recombinant bacterium, and belongs to the technical field of bioengineering. A tannase gene sequence TanLpl from lactobacillus plantarum ATCC14917T is connected with a shuttle plasmid pHT43 with a strong promoter and then is transferred into bacillus subtilis WB600 to prepare recombinant bacteria with high yield of tannase, the enzyme activity reaches 461.12 U/mL under the optimal condition, and efficient expression of tannase in the bacteria is realized; the high-yield tannase recombinant bacteria prepared by the invention can stably inherit carried target genes, and compared with a CK blank group, when the recombinant bacteria are used for fermenting caragana microphylla to prepare caragana microphylla feed, the total phenol content in the caragana microphylla is reduced by 41.33%, the hydrolyzed tannin content is reduced by 70.84%, and the condensed tannin content is reduced by 35.42%; the high-yield tannase recombinant bacterium can improve the tannin degradati