Tissue culture method for limonium bicolor

The invention provides a limonium bicolor tissue culture method. The limonium bicolor tissue culture method comprises the following steps: S1, selection and sterilization of an explant: taking a non-flowering inflorescence of limonium bicolor for disinfection and sterilization; s2, callus induction:...

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Hauptverfasser: FU NANA, LIU YINGJUN, YUAN FENG, ZHAO HUIMIN, MA CAI'E, JIA ZHENYU, LIU YALING, MA HUAILIN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides a limonium bicolor tissue culture method. The limonium bicolor tissue culture method comprises the following steps: S1, selection and sterilization of an explant: taking a non-flowering inflorescence of limonium bicolor for disinfection and sterilization; s2, callus induction: cutting the disinfected and sterilized inflorescence which does not bloom into small sections of 0.8-1.2 cm, putting the small sections on a primary culture medium, and performing induction for 15-20 days to obtain calluses; s3, subculture multiplication culture: inoculating the callus onto a multiplication and differentiation culture medium, and culturing for 15-25 days to obtain adventitious buds; s4, rooting culture: transferring the adventitious buds into a rooting culture medium, and carrying out rooting culture for 5-7 days to obtain aseptic seedlings; according to the method disclosed by the invention, common tissue culture medicaments are screened, medicaments suitable for being respectively required in a