Multistage full liquid phase immunoassay method based on alternating current magnetization response of superparamagnetic nanoparticles

The invention relates to a multistage full liquid phase immunoassay method based on alternating current magnetization response of superparamagnetic nanoparticles, and belongs to the technical field of biological detection. The multi-stage full-liquid-phase immunoassay method provided by the invention is a brand-new three-stage superparamagnetic sensing detection structure, interference caused by non-specific factors such as whole blood can be eliminated through the reaction of the reagent 1 and the reagent 2, and full-liquid-phase superparamagnetic sensing signals are amplified and detected through the reaction of the reagent 2 and the reagent 3. According to the technical effects, the traditional advantages (completely free of separation, high in detection speed and simple to use) of a full-liquid-phase superparamagnetic sensitive detection technology are reserved, interference caused by most of whole blood is eliminated, signal amplification is carried out through a biotin avidin system, the detection sensi