Construction method and application of HPLC (High Performance Liquid Chromatography) characteristic chromatograms of walnut kernel medicinal materials, decoction pieces, standard decoction and formula granules

The invention belongs to the technical field of detection, and particularly relates to a construction method and application of HPLC (High Performance Liquid Chromatography) characteristic chromatograms of walnut kernel medicinal materials, decoction pieces, standard decoction and formula granules....

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Bibliographische Detailangaben
Hauptverfasser: ZHOU HOUCHENG, YANG LIAN, HUANG YU, ZHOU JINGWEI, LUO MANJUAN, FEI WENBO, PENG WENCHEN, MAO QING, LIN BINGXUE, ZHANG LIN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the technical field of detection, and particularly relates to a construction method and application of HPLC (High Performance Liquid Chromatography) characteristic chromatograms of walnut kernel medicinal materials, decoction pieces, standard decoction and formula granules. The HPLC characteristic chromatogram construction method provided by the invention comprises the following steps: preparing a test sample, wherein the test sample is a walnut kernel medicinal material, a walnut kernel decoction piece, a walnut kernel standard decoction or walnut kernel formula granules; dissolving the test article to obtain a test article solution; determining the test sample solution by adopting a high performance liquid chromatography to obtain an HPLC characteristic chromatogram of a corresponding test sample; chromatographic conditions of the high performance liquid chromatography are as follows: a chromatographic column is a C18 column; a mobile phase A is acetonitrile, a mobile phase B is a 0