Space group imaging system and method based on phase separation

The invention relates to the technical field of bioengineering, in particular to a space group imaging system and method based on phase separation. According to the method, multiple rounds of fluorescence imaging, magnetic particle imaging and living cell imaging are combined, accurate quantitative analysis of the subcellular horizontal phase separation process is achieved, and the sensitivity and the resolution ratio of a traditional method are improved. The method is combined with novel spatial proteomics imaging, compared with a traditional low-flux method, a series of spatial proteomics and spatial transcriptomics information in the phase separation process is supplemented, the method can be applied to drug effect evaluation and screening of drug molecules at the cellular level, and the test and development cost is saved. 本发明涉及生物工程技术领域，尤其涉及一种基于相分离的空间组学成像系统及方法。本发明结合多轮荧光成像、磁粒子成像和活细胞成像，实现亚细胞水平相分离过程精确量化分析，改善传统方法的灵敏度和分辨率。本发明结合新型空间组学成像，相比传统低通量方法补充了相分离过程中一系列空间蛋白组学和空间转录组学信息，可应用于药物分子在细胞水平的药效评估和筛选，节约测试和开发成本。