Primer and probe for detecting acipenser dabryanus eDNA and detection method

The invention relates to the field of molecular biology and molecular ecology, and particularly discloses a primer and a probe for detecting acipenser dabryanus eDNA, by designing a specific acipenser dabryanus RPA primer and probe combination and adopting a recombinase polymerase amplification and...

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Bibliographische Detailangaben
Hauptverfasser:	LING LANXIN, WANG HUIFANG, LIANG LINYAN, LI CHENHONG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to the field of molecular biology and molecular ecology, and particularly discloses a primer and a probe for detecting acipenser dabryanus eDNA, by designing a specific acipenser dabryanus RPA primer and probe combination and adopting a recombinase polymerase amplification and lateral flow chromatography test strip (RPA-LFD) detection technology, the acipenser dabryanus eDNA can be rapidly and conveniently detected, the sensitivity is high, the detection time is short, the detection time is short, and the detection efficiency is high. Therefore, field real-time monitoring of the acipenser dabryanus is realized. 本发明涉及分子生物学和分子生态学领域，具体公开了一种检测达氏鲟eDNA的引物和探针，通过设计特异性的达氏鲟的RPA引物和探针组合，采用重组酶聚合酶扩增结合侧流层析试纸条(RPA-LFD)检测技术，能够实现快速、便捷地检测达氏鲟eDNA，并且灵敏高，特异强，从而实现野外实时监测达氏鲟。