G6PD enzyme activity detection kit and preparation method thereof

The invention discloses a G6PD enzyme activity detection kit and a preparation method thereof.The detection kit comprises a reaction tube and a detection card, the reaction tube contains a detection reagent, and the detection reagent contains D-glucose-6-disodium phosphate, nicotinamide adenine dinucleotide phosphate, phenazine methyl sulfate and tetrazole blue; the detection card comprises a panel, a combination pad, an adsorption pad and a bottom plate which are sequentially arranged in a stacked mode, the panel is further provided with a sample adding hole, the sample adding hole is located above the combination pad, the combination pad is configured to intercept reacted color developing substances and nucleic acid in a to-be-detected blood sample, and the adsorption pad is configured to adsorb liquid and hemoglobin in the to-be-detected blood sample. According to the method and the device, interference of hemoglobin interference on result interpretation can be eliminated, and result interpretation is perf