Construction method of high-activity zearalenone degrading enzyme in cattle stomach environment

The invention discloses a construction method of a high-activity zearalenone degrading enzyme in a cattle stomach environment. A zearalenone degrading enzyme mutant takes a zearalenone degrading enzyme with an amino acid sequence as shown in SEQ ID NO.1 as a parent, threonine, threonine, leucine, leucine and leucine are inserted between the 33rd site and the 34th site of the parent, and the zearalenone degrading enzyme mutant is obtained. And a serine, a lysine, a serine, a serine, a leucine and a leucine are inserted between the 145 < th > site and the 146 < th > site. According to the technical scheme provided by the invention, the molecular structure of the zearalenone degrading enzyme is modified by virtue of biotechnologies of site-directed mutagenesis and insertion of a fragment sequence, so that the zearalenone degrading enzyme mutant is obtained, and the mutant has higher enzyme activity in an acidic environment with the pH value of about 6.0, and can be used for preparing the zearalenone degrading en