Transaminase and application thereof in production of sitagliptin intermediate

The invention relates to the technical field of enzyme catalysis, in particular to transaminase and application of the transaminase in production of a sitagliptin intermediate, and the amino acid sequence of the transaminase is shown as SEQ ID NO: 3. The transaminase disclosed by the invention can be used for catalyzing the 1-piperidine-4-(2, 4, 5-trifluorophenyl)-1, 3-dibutanone to generate the sitagliptin intermediate (R)-3-amino-1-piperidine-4-(2, 4, 5-trifluorophenyl)-1-butanone. Compared with wild transaminase, the transaminase provided by the invention not only has higher enzyme activity determination, but also has higher substrate conversion rate and product concentration under specific reaction conditions (50-55 DEG C, pH 9.0-9.5). 本发明涉及酶催化技术领域，尤其是一种转氨酶及其在生产西他列汀中间体的应用，所述转氨酶的氨基酸序列如SEQ ID NO：3所示。本发明的转氨酶可用于催化1-哌啶-4-(2,4,5-三氟苯基)-1,3-二丁酮生成西他列汀中间体(R)-3-氨基-1-哌啶-4-(2,4,5-三氟苯基)-1-丁酮。本发明的转氨酶与野生型转氨酶相比，不仅酶活测定更高，并且在特定反应条件下(50℃-55℃，pH 9.0-9.5)具有更高的底物转化率和产物浓度。