Bionic moist double-layer artificial skin based on silk and collagen and preparation method of bionic moist double-layer artificial skin

The invention discloses bionic moist double-layer artificial skin based on silk and collagen and a preparation method thereof.The bionic moist double-layer artificial skin is of a double-layer structure which simulates an epidermal layer and a corium layer of natural skin respectively, the two-layer structure is compounded through chemical crosslinking, the epidermal layer is composed of a silk fibroin film from silk and has a compact and smooth morphology, and the corium layer is composed of a silk fibroin film from collagen. The tensile modulus is about 40 MPa, and the tensile and protective effects can be provided; the corium layer is composed of collagen hydrogel, has loose and porous morphology, and can provide space for cell adhesion and growth. Collagen hydrogel of the corium layer has the effect of promoting cell adhesion and proliferation, and the corium layer can be rapidly regenerated. Therefore, the double-layer artificial skin provided by the invention not only can be used for treating skin defec