Internal reference gene, primer probe and detection kit for exosome RNA detection

The invention discloses a reference gene, a primer probe and a detection kit for exosome RNA (Ribonucleic Acid) detection. The reference gene used for exosome RNA detection provided by the invention is an HSP70 gene. A primer sequence for detecting the reference gene HSP70 is shown as SEQ ID NO: 1 and SEQ ID NO: 2, and a probe sequence is shown as SEQ ID NO: 3. The reference gene HSP70 provided by the invention can be stably detected in blood plasma exosomes of healthy people and cancer patients, is slightly influenced by the types of blood collection tubes, and can also be detected in cryopreserved blood plasma, so that the reference gene HSP70 can be used as the reference gene for exosome detection. When the kit is used for exosome EML4-ALK fusion gene detection, the reference gene HSP70 is an mRNA sequence, and reverse transcription and multiple RT-PCR can be carried out together with target detection. 本发明公开了用于外泌体RNA检测的内参基因及引物探针和检测试剂盒。本发明提供的用于外泌体RNA检测的内参基因为HSP70基因。检测内参基因HSP70的引物序列如SEQ ID NO:1和SEQ ID NO:2所示