Method for cell-free synthesis of lycopene based on utilization path of isopentenol

The invention discloses a method for cell-free synthesis of lycopene based on an isopentenol utilization pathway, which comprises the following steps: constructing seven key recombinant plasmids for synthesizing lycopene in an expression IUP pathway, and carrying out induced expression and ultrasonication in escherichia coli to obtain an escherichia coli cell lysis solution rich in key enzymes. The lycopene is efficiently synthesized by constructing an acellular metabolism engineering system in vitro by exogenously adding a substrate, energy, cofactors and the like required by a synthesis reaction. According to the method disclosed by the invention, the isopentenol is combined with cell-free metabolic engineering to be applied to in-vitro synthesis of the lycopene for the first time, only two-step phosphorylation reaction is used for replacing the original more than seven-step MEP and MVA pathways to synthesize precursor substances IPP and DMAPP of the lycopene, the metabolic pathway is greatly shortened, the