Preparation and application of lipid intercalation modified mitochondria

The invention discloses preparation and application of lipid intercalation modified mitochondria. The lipid intercalation modified mitochondria disclosed by the invention comprises an in-vitro source mitochondria, and a targeting-response lipid material is intercalated on the surface of the in-vitro source mitochondria; the targeting-response lipid material is formed by connecting a hydrophobic tail part, a pathological microenvironment responsive connecting bond and a targeting group, the hydrophobic tail part is embedded in an in-vitro source mitochondrial membrane, and the targeting group and a cross-linking agent are mutually cross-linked to form a shell which wraps in-vitro source mitochondria. The in-vitro mitochondria is subjected to intercalation modification by utilizing a lipid component with a targeting group and a responsive connecting bond, and a wrapping shell is formed on the surface of the in-vitro mitochondria, so that not only can the activity of the in-vitro mitochondria be maintained, but