Recombinant yeast cells

Disclosed is a recombinant yeast cell functionally expressing: a nucleic acid sequence encoding a protein having glycerol dehydrogenase activity; a nucleic acid sequence encoding a protein having dihydroxyacetone kinase activity; and-a nucleic acid sequence encoding a protein having glycerol transporter activity wherein the expression of the nucleic acid sequence encoding the protein having glycerol transporter activity is controlled by the promoter ("GT promoter"), the anaerobic/aerobic expression ratio of the GT promoter to the glycerol transporter being 2 or higher; and a method for producing ethanol using such recombinant yeast cells. 公开了一种重组酵母细胞，该重组酵母细胞功能性地表达：-编码具有甘油脱氢酶活性的蛋白质的核酸序列；-编码具有二羟基丙酮激酶活性的蛋白质的核酸序列；和-编码具有甘油转运蛋白活性的蛋白质的核酸序列，其中编码具有甘油转运蛋白活性的该蛋白质的该核酸序列的表达在启动子("GT启动子")的控制下，该GT启动子对甘油转运蛋白的厌氧/好氧表达比率为2或更高；以及一种使用这种重组酵母细胞生产乙醇的方法。