Detection method of etomidate medicine and application thereof

The invention discloses a detection method and application of etomidate drugs, and relates to the technical field of analysis and detection. The detection method comprises the following steps: dissolving a to-be-detected sample with a first dissolving solvent to obtain a sample solution; dissolving an etomidate reference substance, an etomidate acid reference substance, a medetomidate reference substance, a methyl etomidate reference substance and a 2-sulfydryl etomidate reference substance with a second dissolving solvent to obtain a reference substance solution; and detecting the sample solution and the reference substance solution by adopting an ultra-high performance liquid chromatograph-mass spectrometer to obtain a spectrogram, and determining whether the sample to be detected contains etomidate, etomidate acid, medetomidate, methyl etomidate and/or 2-mercapto etomidate according to the spectrogram. The detection method provided by the invention can rapidly realize qualitative and quantitative detection