Gn region subunit vaccine of SEOV as well as preparation method and application of Gn region subunit vaccine

The invention relates to the field of vaccine preparation methods, and particularly discloses a Gn region subunit vaccine of SEOV as well as a preparation method and application thereof, and the subunit vaccine is prepared by the following steps: respectively extracting consensus sequences of M gene sequences of Hantaan virus and Seoul virus, removing retention signals in the consensus sequences, and respectively expressing soluble proteins with different lengths. Carrying out protein purification by a method of adding an HIS tag to a sequence C segment, cloning the synthesized gene into an expression vector, transfecting an S2 cell, and screening a high-expression strain; and then carrying out batch production, and carrying out protein purification through the HIS tag carried by the protein to obtain the general subunit vaccine. The subunit vaccine prepared by the invention is good in stability, easy to purify, free of viral nucleic acid components and relatively high in safety, and is a universal vaccine ca