Application of UGT33F32 gene as target spot in prevention and control of spodoptera frugiperda

The invention belongs to the field of pest control and relates to application of a UGT33F32 gene as a target spot in control of spodoptera frugiperda. According to the invention, a major agricultural pest spodoptera frugiperda is taken as a research object, a UGT33F32 gene of the spodoptera frugiperda is taken as a molecular target, a CRISPR/Cas9 gene editing technology is combined, a UGT33F32 mutant of the spodoptera frugiperda is constructed, after the UGT33F32 gene is knocked out by bioassay, the sensitivity of the spodoptera frugiperda to a main insect-resistant compound dibutran of corn is remarkably improved, and the UGT33F32 gene of the spodoptera frugiperda is knocked out. The 7-day death rate on corn is obviously increased, the developmental duration of larvae is obviously prolonged, the pupation rate and the pupa weight are obviously reduced, and no next-generation individual is generated. The invention provides a Spodoptera frugiperda gene knockout technology system and a Spodoptera frugiperda UGT3