Method for detecting enzyme activity of human cytochrome P450 3A4 and application of method

The invention provides a method for detecting enzymatic activity of human cytochrome P4503A4 and application of the human cytochrome P4503A4. The structural general formula of the human cytochrome P4503A4 is as shown in formula (1): # imgabs0 # R is H, methyl or isopropyl. The invention develops a CYP3A4 specific fluorescent substrate, the CYP3A4 specific fluorescent substrate can be specifically catalyzed by CYP3A4 under physiological conditions to generate a hydroxylation reaction and generate a single 4-hydroxylation product, and the activity of CYP3A4 in a biological sample can be quantitatively detected by detecting the elimination of the substrate in unit time or the generation amount of the 4-hydroxylation product. A CYP3A4 activity quantitative detection method constructed by virtue of the fluorescent substrate can be used for determining the activity of CYP3A4 in biological samples such as CYP3A4 recombinant single enzyme, cell or tissue preparations and the like so as to carry out quality control on