Collagen matrix biological membrane preparation method, collagen matrix biological membrane and application of collagen matrix biological membrane

The invention relates to a preparation method of a collagen matrix biological membrane, the collagen matrix biological membrane and application of the collagen matrix biological membrane, and the preparation method of the collagen matrix biological membrane comprises the following steps: S1, preparing an acellular matrix layer with a compact structure; s2, preparing a collagen suspension; s3, extruding and injecting the collagen suspension into a mold for molding, wetting the acellular matrix layer, flatly laying the wetted acellular matrix layer on the surface of the collagen suspension, lightly pressing to extrude bubbles, and standing to enable the wetted acellular matrix layer and the collagen suspension to be tightly combined; s4, freeze-drying the closely combined acellular matrix layer and collagen suspension to obtain a composite freeze-dried layer; and S5, carrying out physical crosslinking on the composite freeze-drying layer to obtain the collagen matrix biological membrane. The collagen matrix bio