Carrier preparation method

The present disclosure provides methods of viral vector preparation and purification. In particular, the present disclosure provides improved methods for the preparation of lentiviral vectors from host cells grown in a suspension. More specifically, the present disclosure provides an improved large scale lentivirus preparation method, the large scale lentivirus preparation method comprising: growing cells to a suitable number; carrying out transfection by using a lentivirus packaging plasmid, a transfer plasmid and a transfection agent; carrying out treatment by using endonuclease; collecting and clarifying supernatant of the suspension culture; capturing and concentrating the lentiviral vector using affinity chromatography or cation exchange chromatography; filtering the concentrated lentiviral vector; performing ultrafiltration and percolation on the lentiviral vector; blending the lentiviral vector; and performing sterile filtration on the blended bulk lentiviral vector. 本公开提供了病毒载体制备和纯化方法。具体地讲，本公开提供了用于由在悬浮