Liquid chromatography-tandem mass spectrometry detection method for six artificial sweeteners in fruits
The invention discloses a liquid chromatography-tandem mass spectrometry detection method for six artificial sweeteners in fruits, which comprises the following steps: (1) homogenizing and crushing citrus, jujube, strawberry or grape samples by using a homogenizer, putting into a subpackaging contai...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a liquid chromatography-tandem mass spectrometry detection method for six artificial sweeteners in fruits, which comprises the following steps: (1) homogenizing and crushing citrus, jujube, strawberry or grape samples by using a homogenizer, putting into a subpackaging container, and freezing and preserving for later use; (2) weighing a sample, putting the sample into a centrifugal tube, adding an extraction solvent into the centrifugal tube filled with the sample by adopting acetonitrile water as the extraction solvent, shaking for 1 minute, and centrifuging for 15 minutes under the condition of 8000 r/min; the volume percent concentration of acetonitrile in the acetonitrile water is 10%, formic acid is added into the acetonitrile water, and the volume percent concentration of the formic acid in the acetonitrile water is 0.05%; the mass-volume ratio of the sample to the extraction solvent is 1: 5, and the unit of the mass-volume ratio is g/mL; (3) filtering the centrifuged supernate w |
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