Primer probe combination, kit and method for multiple fluorescent PCR detection for identifying mycoplasma gallisepticum vaccine strain

The invention discloses a primer probe combination, a kit and a method for multiple fluorescent PCR (polymerase chain reaction) detection for identifying mycoplasma gallisepticum vaccine strains. The invention firstly discloses a primer probe combination for multiple fluorescent PCR detection for id...

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Bibliographische Detailangaben
Hauptverfasser:	ZHANG PING, WANG GUOXING, CHEN XIANGYONG, XIN WEN, GENG LIANG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a primer probe combination, a kit and a method for multiple fluorescent PCR (polymerase chain reaction) detection for identifying mycoplasma gallisepticum vaccine strains. The invention firstly discloses a primer probe combination for multiple fluorescent PCR detection for identifying mycoplasma gallisepticum vaccine strains, and the primer probe combination comprises primer probes for mycoplasma gallisepticum TS-11, F, 6/85 and R vaccine strains. The invention further discloses a kit containing the primer probe combination and a method for simultaneously identifying mycoplasma gallisepticum TS-11, F, 6/85 and R vaccine strains. The method for identifying the mycoplasma gallisepticum TS-11, F, 6/85 and R vaccine strains is high in sensitivity and strong in specificity; through a single-tube quadruple detection mode, detection results of four vaccine strains are directly obtained after amplification, the detection process is short, the detection efficiency is high, the detection rate is