Primer pair and probe for quantitative analysis of DNA residue and application of primer pair and probe

The invention aims to provide a method for detecting DNA residues, in particular to a method for quantitatively detecting residues of VSVG, E1A and SV40, the method can quantitatively detect lentivirus transduction DNA residues and host genome DNA residues in a short time, multiple quantitative detection of three genes is realized, expensive instruments and equipment are not needed, the specificity is high, the linear range is wide, and the method is suitable for industrial production. And the method has the advantages of high sensitivity, high reference value for quality control release detection of the CAR-T cell preparation, shortening of the CAR-T quality control time, and suitableness for popularization and application. 本发明目的是提供一种检测DNA残留，具体为对VSVG、E1A和SV40进行残留定量检测的方法，该方法能够在较短的时间内定量检测出慢病毒转导性DNA残留和宿主基因组DNA残留，实现3种基因的多重定量检测，且不需要昂贵的仪器设备，专属性较高、线性范围较宽，且灵敏度较高，对CAR-T细胞制剂的质量控制放行检测具有较高的参考价值，可以缩短CAR-T质控时间，适于推广应用。