Nilaparvata lugens NlRan gene RNAi expression vector and application thereof

The invention discloses a nilaparvata lugens NlRan gene RNAi expression vector and application thereof, and belongs to the field of bioengineering, and the nilaparvata lugens NlRan gene RNAi expression vector comprises the following steps: (1) constructing a target nilaparvata lugens Ran gene RNAi e...

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Hauptverfasser: YANG LING, LIAN JINJIN, ZHANG WEILIN, ZHANG JINSHI, QIAN YILU
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a nilaparvata lugens NlRan gene RNAi expression vector and application thereof, and belongs to the field of bioengineering, and the nilaparvata lugens NlRan gene RNAi expression vector comprises the following steps: (1) constructing a target nilaparvata lugens Ran gene RNAi expression vector; and (2) carrying out genetic transformation on rice by using the RNAi expression vector, so that the rice obtains brown planthopper resistance. The RNAi expression vector disclosed by the invention has the characteristics of high efficiency, specificity, relatively high biological safety, environmental friendliness and the like, and the brown planthopper resistance of rice can be remarkably improved. 本发明公开了褐飞虱NlRan基因RNAi表达载体及其应用,属于生物工程领域,(1)构建靶向褐飞虱Ran基因的RNAi表达载体;(2)将RNAi表达载体遗传转化水稻,从而使水稻获得褐飞虱抗性。本发明RNAi表达载体具有高效性、特异性及较高的生物安全性和对环境友好等特点,可以显著提高水稻的褐飞虱抗性。