Method for improving and evaluating antioxidant and anti-inflammatory activity of tortoise shells

A method for improving and evaluating oxidation resistance and anti-inflammatory activity of tortoise shells comprises the following steps: step 1, treating the tortoise shells at 110-130 DEG C for 60-200 minutes, then drying the tortoise shells to constant weight at 55-65 DEG C, and then grinding t...

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Bibliographische Detailangaben
Hauptverfasser: REN MENGYING, LAI NIANYUE, LIN LIN, LU JIANFENG, LEE JUNG YOUNG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:A method for improving and evaluating oxidation resistance and anti-inflammatory activity of tortoise shells comprises the following steps: step 1, treating the tortoise shells at 110-130 DEG C for 60-200 minutes, then drying the tortoise shells to constant weight at 55-65 DEG C, and then grinding the tortoise shells into powder to obtain tortoise shell powder; 2, respectively detecting dissolution conditions of water-soluble components of the tortoise shell powder, wherein the dissolution conditions comprise the content of water-soluble extract, the content of water-soluble protein and the content of peptide; step 3, adding the tortoise shell powder into distilled water, heating for 0.5-1.5 hours in a water bath at 55-65 DEG C, and repeating for 2-4 times; concentrating the supernate, storing at-20 DEG C, and processing into freeze-dried powder; and 4, detecting the antioxidant activity and the anti-inflammatory activity of the freeze-dried powder by adopting a DPPH free radical scavenging kit, an ABTS free