Method for rapidly collecting pollen in pigment marigold seed production process

The invention relates to a method for rapidly collecting pollen in a pigment marigold seed production process. The method comprises the following steps: (1) seed pretreatment; (2) obtaining sterile seedlings; (3) adventitious bud induction culture: cutting leaves of the obtained aseptic seedlings, a...

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Hauptverfasser: YANG XIUMEI, SU YAN, ZOU LING, HUANG WANGQI, ZHANG YIPING, LEI ZHENGSONG, CHEN XIUHUA, XU FENG, MENG CHAO, JIANG YALIAN, QIAN SHAOFANG, WANG LIHUA, ZHAO AXIANG, NIU SHANCE, ZHANG LIFANG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to a method for rapidly collecting pollen in a pigment marigold seed production process. The method comprises the following steps: (1) seed pretreatment; (2) obtaining sterile seedlings; (3) adventitious bud induction culture: cutting leaves of the obtained aseptic seedlings, and inoculating the cut leaves to an adventitious bud induction culture medium for culture; (4) blooming adventitious buds, and collecting pollen; (5) subculture; cutting off the roots of the rooted seedlings after pollen collection, transferring the rooted seedlings into a new culture medium for continuous subculture, and collecting pollen while enabling the rooted seedlings to continuously bloom. The method is rapid and simple, a large number of neat and uniform seedlings with consistent purity are obtained in a short period based on tissue culture, so that pollen is conveniently, rapidly and efficiently collected, the activity of the obtained pollen is still high during subculture, the breeding process is improve