Kit for determining troponin I by homogeneous chemiluminescence method, determination method and application

The invention belongs to the technical field of in-vitro detection, and relates to a kit for determining troponin I by a homogeneous chemiluminescence method, a determination method and application. The kit comprises a reagent R1, a reagent R2 and a photosensitive liquid, the reagent R1 is a luminou...

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Hauptverfasser: XU XIAOJING, HUANG JINFA, SHANG YIWEN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the technical field of in-vitro detection, and relates to a kit for determining troponin I by a homogeneous chemiluminescence method, a determination method and application. The kit comprises a reagent R1, a reagent R2 and a photosensitive liquid, the reagent R1 is a luminous microsphere coated with a cTnI antibody; the reagent R2 is a cTnI antibody labeled by biotin; the photosensitive liquid is a photosensitive microsphere coated with streptavidin; the luminous microspheres are carboxylated magnetic beads filled with lanthanide series metal chelates; the photosensitive microspheres are carboxylated magnetic beads filled with a metal phthalocyanine compound or a derivative of the metal phthalocyanine compound. The kit is low in detection limit, wide in linear detection range, high in accuracy and repeatability, small in batch difference, high in analysis specificity and excellent in comprehensive performance, troponin I can be effectively detected, and the kit is widely applied to no