Method for detecting antibiotics in fishery Chinese herbal medicine powder

The invention discloses a method for detecting antibiotics in fishery Chinese herbal medicine powder, which comprises the following steps: (1) extracting Chinese herbal medicine powder to be detected by using an extraction solvent to obtain a Chinese herbal medicine extract; (2) adsorbing interference impurities in the Chinese herbal medicine extract by using a purifying agent to obtain a test solution; (3) carrying out quantitative analysis on the content of the antibiotics in the test solution by adopting a high performance liquid chromatography-tandem mass spectrometry method; wherein the extraction solvent is 0.1% formic acid acetonitrile, and the purifying agent is an octadecyl silica gel bonded phase. The method for detecting the quinolone antibiotics and the sulfonamide antibiotics in the fishery Chinese herbal medicine powder is established on the basis of QuEChERS in combination with high performance liquid chromatography-tandem mass spectrometry, has the characteristics of simple pretreatment, rapid