Carbonyl reductase mutant and application thereof in preparation of (S)-glassine

The invention belongs to the field of bioengineering, and particularly relates to a carbonyl reductase mutant and application thereof in preparation of (S)-vitrin. According to the invention, carbonyl reductase CoCR13 of Candida orthopsilosCo is taken as a source for enzyme modification, and a mutan...

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Hauptverfasser: CUI XILI, MA QINYUAN, XU KE, GUAN XIBO, SHANG JING, WANG HUI, LAN JIANG, WANG BIN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the field of bioengineering, and particularly relates to a carbonyl reductase mutant and application thereof in preparation of (S)-vitrin. According to the invention, carbonyl reductase CoCR13 of Candida orthopsilosCo is taken as a source for enzyme modification, and a mutant of the carbonyl reductase CoCR13 is screened, so that a key residue influencing the enzymatic property of the initial carbonyl reductase is determined, and meanwhile, the carbonyl reductase mutant with high activity is obtained. The carbonyl reductase mutant obtained through screening has good catalytic efficiency in the reaction of catalyzing 1-C-(beta-D-xylopyranosyl)-acetone to prepare the (S)-glassine, the catalytic efficiency ranges from 1.0 mM S to 1.7 mM S , meanwhile, the optical purity of the obtained (S)-glassine is high, and the ee value ranges from 99.4% to 99.9%. 本发明属于生物工程领域,具体涉及羰基还原酶突变体及其在制备(S)-玻色因中的应用。本发明以假丝酵母菌CandidaorthopsilosisCo的羰基还原酶CoCR13为来源进行酶的改造,并对其突变体进行了筛选,确定了影响初始羰基还