Escherichia coli recombinant strain for synthesizing phycocyanobilin and application

The invention discloses an escherichia coli recombinant strain for synthesizing phycocyanobilin and application, and belongs to the technical field of bioengineering. According to the invention, source screening and rational transformation of the phycocyanobilin ferredoxin reductase (PcyA) are realized, and the problem of low yield of phycocyanobilin in the prior art is solved. A key rate-limiting enzyme which is most suitable for biliverdin and phycocyanobilin escherichia coli synthesis is screened from PcyA from five different algae sources, and rational transformation is carried out on the basis, so that high-efficiency synthesis of shake flask level phycocyanobilin in escherichia coli can be realized. At the shake flask level, the yield of the obtained phycocyanobilin can reach 24.80 mg/L, and the result lays a foundation for application of the phycocyanobilin in the fields of food, medicine and cosmetics. 本发明公开了一种合成藻蓝素的大肠杆菌重组菌株及应用，属于生物工程技术领域。本发明实现了藻蓝素铁氧还蛋白还原酶(PcyA)来源筛选和理性改造，解决了之前藻蓝素产量低的问题。通过从5种不同藻类来源的Pcy