Ratio-dependent fluorescent probe, preparation method thereof and application of ratio-dependent fluorescent probe in fluorescence detection of serotonin
The invention discloses a ratiometric fluorescent probe, a preparation method thereof and application of the ratiometric fluorescent probe in fluorescence detection of serotonin. The ratiometric fluorescent probe is an MQDs-Eu < 3 + >-DPA fluorescent probe. The MQDs-Eu < 3 + >-DPA fluore...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a ratiometric fluorescent probe, a preparation method thereof and application of the ratiometric fluorescent probe in fluorescence detection of serotonin. The ratiometric fluorescent probe is an MQDs-Eu < 3 + >-DPA fluorescent probe. The MQDs-Eu < 3 + >-DPA fluorescent probe is formed by complexing MQDs, Eu < 3 + > and DPA. The MQDs which are non-toxic, green and environment-friendly and emit blue fluorescence, Eu < 3 + > and DPA are complexed to form the MQDs-Eu < 3 + >-DPA fluorescent probe capable of emitting blue and red fluorescence at the same time, the MQDs not only serve as a reference material, but also have a photocatalysis effect, and by means of the internal filtration effect between 5-HT and the MQDs-Eu < 3 + >-DPA and the photocatalysis effect of the MQDs, the fluorescence intensity of the fluorescent probe is improved, and the fluorescence intensity of the fluorescent probe is improved. The red fluorescence of Eu < 3 + > in the MQDs-Eu < 3 + >-DPA probe is quenched when |
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