Preparation method of personalized mRNA (messenger ribonucleic acid) vaccine for targeted liver tumor
The invention provides a preparation method of a targeted liver tumor personalized mRNA vaccine, and belongs to the technical field of medical preparations. The preparation method comprises the following steps: determining a mutant polypeptide, primarily screening the mutant polypeptide, screening a...
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| creator | ZHANG CHUANPENG XU MIAO QIANG BANGMING WANG LIXIN FENG JIANHAI LIU MINGLU JIN HAIFENG HAN QINGMEI LI SHUHONG |
| description | The invention provides a preparation method of a targeted liver tumor personalized mRNA vaccine, and belongs to the technical field of medical preparations. The preparation method comprises the following steps: determining a mutant polypeptide, primarily screening the mutant polypeptide, screening a high-affinity peptide fragment, integrating to obtain a neoantigen polypeptide, obtaining a neoantigen polypeptide with strong immunogenicity, synthesizing mRNA, and preparing the mRNA vaccine. According to the invention, mutant polypeptides can be obtained by sequencing tumor patients, high-affinity neoantigen polypeptides can be obtained by screening, neoantigen polypeptides with strong immunogenicity can be obtained by further screening, and then the neoantigen polypeptides are connected by adopting a specific connection mode to prepare personalized tumor mRNA vaccines; the mRNA vaccine can stimulate CD8 + T cells, so that the expression of IFN-gamma and TNF-alpha of the CD8 + T cells is up-regulated, and the s |
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The preparation method comprises the following steps: determining a mutant polypeptide, primarily screening the mutant polypeptide, screening a high-affinity peptide fragment, integrating to obtain a neoantigen polypeptide, obtaining a neoantigen polypeptide with strong immunogenicity, synthesizing mRNA, and preparing the mRNA vaccine. According to the invention, mutant polypeptides can be obtained by sequencing tumor patients, high-affinity neoantigen polypeptides can be obtained by screening, neoantigen polypeptides with strong immunogenicity can be obtained by further screening, and then the neoantigen polypeptides are connected by adopting a specific connection mode to prepare personalized tumor mRNA vaccines; the mRNA vaccine can stimulate CD8 + T cells, so that the expression of IFN-gamma and TNF-alpha of the CD8 + T cells is up-regulated, and the s</description><language>chi ; eng</language><subject>CHEMISTRY ; HUMAN NECESSITIES ; HYGIENE ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; MEDICAL OR VETERINARY SCIENCE ; METALLURGY ; ORGANIC CHEMISTRY ; PEPTIDES ; PHYSICS ; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES ; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS ; TESTING</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240416&DB=EPODOC&CC=CN&NR=117883558A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76293</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240416&DB=EPODOC&CC=CN&NR=117883558A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>ZHANG CHUANPENG</creatorcontrib><creatorcontrib>XU MIAO</creatorcontrib><creatorcontrib>QIANG BANGMING</creatorcontrib><creatorcontrib>WANG LIXIN</creatorcontrib><creatorcontrib>FENG JIANHAI</creatorcontrib><creatorcontrib>LIU MINGLU</creatorcontrib><creatorcontrib>JIN HAIFENG</creatorcontrib><creatorcontrib>HAN QINGMEI</creatorcontrib><creatorcontrib>LI SHUHONG</creatorcontrib><title>Preparation method of personalized mRNA (messenger ribonucleic acid) vaccine for targeted liver tumor</title><description>The invention provides a preparation method of a targeted liver tumor personalized mRNA vaccine, and belongs to the technical field of medical preparations. 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The preparation method comprises the following steps: determining a mutant polypeptide, primarily screening the mutant polypeptide, screening a high-affinity peptide fragment, integrating to obtain a neoantigen polypeptide, obtaining a neoantigen polypeptide with strong immunogenicity, synthesizing mRNA, and preparing the mRNA vaccine. According to the invention, mutant polypeptides can be obtained by sequencing tumor patients, high-affinity neoantigen polypeptides can be obtained by screening, neoantigen polypeptides with strong immunogenicity can be obtained by further screening, and then the neoantigen polypeptides are connected by adopting a specific connection mode to prepare personalized tumor mRNA vaccines; the mRNA vaccine can stimulate CD8 + T cells, so that the expression of IFN-gamma and TNF-alpha of the CD8 + T cells is up-regulated, and the s</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | CHEMISTRY HUMAN NECESSITIES HYGIENE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEDICAL OR VETERINARY SCIENCE METALLURGY ORGANIC CHEMISTRY PEPTIDES PHYSICS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS TESTING |
| title | Preparation method of personalized mRNA (messenger ribonucleic acid) vaccine for targeted liver tumor |
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