Preparation method of personalized mRNA (messenger ribonucleic acid) vaccine for targeted liver tumor

The invention provides a preparation method of a targeted liver tumor personalized mRNA vaccine, and belongs to the technical field of medical preparations. The preparation method comprises the following steps: determining a mutant polypeptide, primarily screening the mutant polypeptide, screening a high-affinity peptide fragment, integrating to obtain a neoantigen polypeptide, obtaining a neoantigen polypeptide with strong immunogenicity, synthesizing mRNA, and preparing the mRNA vaccine. According to the invention, mutant polypeptides can be obtained by sequencing tumor patients, high-affinity neoantigen polypeptides can be obtained by screening, neoantigen polypeptides with strong immunogenicity can be obtained by further screening, and then the neoantigen polypeptides are connected by adopting a specific connection mode to prepare personalized tumor mRNA vaccines; the mRNA vaccine can stimulate CD8 + T cells, so that the expression of IFN-gamma and TNF-alpha of the CD8 + T cells is up-regulated, and the s