Method for creating turnip mosaic virus disease resistant brassica napus new germplasm through gene editing technology and application

The invention discloses a method for creating a novel brassica napus germplasm resistant to turnip mosaic virus through a gene editing technology and application, and relates to the field of plant molecular biology and gene engineering. According to the invention, gene editing is carried out on an exon region of a BneIF2Bbeta gene through a CRISPR-Cas9 technology, and the incomplete conservative property of a DBS endogenous repair mechanism of an organism is utilized, so that the DNA sequence is subjected to frameshift mutation, protein translation is terminated in advance, and a function deletion type material is obtained. The resistance of the material to TuMV is obviously enhanced, and other important horticultural characters are not obviously changed. The new germplasm has great application value in fundamental research and population improvement of a plant anti-TuMV molecular mechanism and breeding work of new germplasm creation. 本发明公开一种通过基因编辑技术创制抗芜菁花叶病毒病甘蓝型油菜新种质的方法及应用，涉及植物分子生物学和基因工程领域。本发明通过CRISPR-Cas9技术