Protein adhesion resistant extracellular vesicle selective separation material and separation method

The invention discloses a protein adhesion resistant extracellular vesicle selective separation material and a separation method. The separation material is prepared by the following steps: firstly, carrying out amyloid phase transformation on bovine serum albumin on the surfaces of silicon dioxide microspheres to form a protein nano-film; and modifying the phospholipid specific recognition peptide on the surface of the protein nano-film through biological coupling reaction. The protein nano-film on the surface of the silicon dioxide microsphere has an anti-adhesion characteristic, so that the adsorption of impurity protein in a biological matrix can be effectively reduced; the phospholipid specific recognition peptide has a specific recognition effect on phospholipid on the surface of an extracellular vesicle membrane, and can selectively recognize target extracellular vesicles. According to the separation material, the separation purity of the extracellular vesicles is improved from the two aspects of reduc