Culture method of stem tip stem cells of tea trees

The invention relates to the technical field of plant stem cell culture, and particularly discloses a culture method of stem tip stem cells of tea trees. The culture method comprises the following steps: step 1, culturing meristem of a stem tip in a stem cell induction culture medium to obtain a ste...

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Bibliographische Detailangaben
Hauptverfasser: ZHAO YONGJUN, ZHOU JIANGSHAN, FANG XIAOXIA, ZHAO YIMING
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention relates to the technical field of plant stem cell culture, and particularly discloses a culture method of stem tip stem cells of tea trees. The culture method comprises the following steps: step 1, culturing meristem of a stem tip in a stem cell induction culture medium to obtain a stem tip stem cell group; step 2, carrying out enzymolysis treatment on the stem tip stem cell cluster by using an enzyme solution containing pectinase and cellulase to remove cell walls, and separating to obtain stem tip stem cells; step 3, culturing the stem tip stem cells for about two weeks, removing cell clusters and large-particle residues, and further carrying out enlarged culture, so as to obtain the stem tip stem cells. The stem cell induction culture medium is prepared from the following components: an MS (Murashige and Skoog) culture medium, 0.4 to 0.8 mg/mL of naphthylacetic acid, 0.4 to 0.8 mg/mL of 6-benzylamino adenine, 0.1 to 0.5 mg/mL of indoleacetic acid and 0.1 to 0.5 mg/mL The stem cells of the ste