Preparation method of Didymela segeticola hypha protoplast

The invention discloses a preparation method of a Didymela segeticola hypha protoplast, and belongs to the technical field of biology. The preparation method of the protoplast comprises the following steps: culturing D.segeticola hyphae for about 7 days, washing the hyphae with sterilized distilled water, and transferring the hyphae into a YEPD liquid culture medium for culturing; and then carrying out enzymolysis on the hyphae by adopting a mixed enzymatic hydrolysate of driselase, lywallzyme and helicase, filtering and centrifuging to obtain the D.segeticola hypha protoplast. The invention shows that the hypha protoplast of D.segeticola can provide an important experimental material for researching the pathogenic mechanism of the strain and creating a bactericide. 本发明公开了一种Didymella segeticola菌丝原生质体的制备方法，属于生物技术领域。原生质体的制备方法包括将D.segeticola菌丝培养约7d，采用灭菌蒸馏水清洗，并转至YEPD液体培养基中培养；然后采用崩溃酶、溶壁酶和蜗牛酶的混合酶解液对菌丝进行酶解、过滤、离心，即得到D.segeticola菌丝原生质体。本发明表明D.segeticola的菌丝原生质体可为研究该菌的致病机制及杀菌剂创制提供重要的实验材料。