DNA-fatty acid conjugate and application thereof in modification of cell membrane
The invention relates to the technical field of cell engineering, in particular to a DNA-fatty acid conjugate and application thereof in cell membrane modification. According to the method, fatty acid is selected as a hydrophobic anchor point, the characteristic of high designability of DNA is combi...
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| creator | LI QIN CHOU FENGLI LI JIN |
| description | The invention relates to the technical field of cell engineering, in particular to a DNA-fatty acid conjugate and application thereof in cell membrane modification. According to the method, fatty acid is selected as a hydrophobic anchor point, the characteristic of high designability of DNA is combined, and a hydrophobic selective cell membrane modification method is developed by regulating and controlling the distance and the length of the fatty acid in a DNA nanostructure. Compared with a traditional nucleic acid probe using cholesterol, the prepared nucleic acid probe based on fatty acid has remarkable pH selectivity in the aspect of cell membrane insertion, and membrane insertion can be achieved under the condition that the pH is 6.5-6.8. Compared with other cell membrane modification methods (such as a gene engineering-based method, a cell metabolism-based method and a covalent cross-linking-based method), the prepared nucleic acid probe has the advantages of rapidness, high efficiency, high universality |
| format | Patent |
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According to the method, fatty acid is selected as a hydrophobic anchor point, the characteristic of high designability of DNA is combined, and a hydrophobic selective cell membrane modification method is developed by regulating and controlling the distance and the length of the fatty acid in a DNA nanostructure. Compared with a traditional nucleic acid probe using cholesterol, the prepared nucleic acid probe based on fatty acid has remarkable pH selectivity in the aspect of cell membrane insertion, and membrane insertion can be achieved under the condition that the pH is 6.5-6.8. 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According to the method, fatty acid is selected as a hydrophobic anchor point, the characteristic of high designability of DNA is combined, and a hydrophobic selective cell membrane modification method is developed by regulating and controlling the distance and the length of the fatty acid in a DNA nanostructure. Compared with a traditional nucleic acid probe using cholesterol, the prepared nucleic acid probe based on fatty acid has remarkable pH selectivity in the aspect of cell membrane insertion, and membrane insertion can be achieved under the condition that the pH is 6.5-6.8. Compared with other cell membrane modification methods (such as a gene engineering-based method, a cell metabolism-based method and a covalent cross-linking-based method), the prepared nucleic acid probe has the advantages of rapidness, high efficiency, high universality</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | ADHESIVES BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF DYES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FORELSEWHERE MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MISCELLANEOUS APPLICATIONS OF MATERIALS MISCELLANEOUS COMPOSITIONS MUTATION OR GENETIC ENGINEERING NATURAL RESINS NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PAINTS PHYSICS POLISHES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS TESTING VINEGAR WINE |
| title | DNA-fatty acid conjugate and application thereof in modification of cell membrane |
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