Method for ultralow-temperature preservation of flemingia macrophylla seeds and improvement of germination rate

The invention discloses an ultralow-temperature storage method of moghania macrophylla seeds, which comprises the following steps: 1) under a dark condition, drying the moghania macrophylla seeds by using a silica gel desiccant until the water content is 5.5-9.5%; and 2) soaking the dried seeds in a...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: WEI YING, CHEN XIAOYING, TAN MEI, LEI MING, LIU HAN, OU XIALIAN, LI CUI, ZHANG ZHANJIANG, HUANG LIRONG, TANG CHUNFENG, GUO XIAOYUN, LI QIAN
Format: Patent
Sprache:chi ; eng
Schlagworte:
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue
container_start_page
container_title
container_volume
creator WEI YING
CHEN XIAOYING
TAN MEI
LEI MING
LIU HAN
OU XIALIAN
LI CUI
ZHANG ZHANJIANG
HUANG LIRONG
TANG CHUNFENG
GUO XIAOYUN
LI QIAN
description The invention discloses an ultralow-temperature storage method of moghania macrophylla seeds, which comprises the following steps: 1) under a dark condition, drying the moghania macrophylla seeds by using a silica gel desiccant until the water content is 5.5-9.5%; and 2) soaking the dried seeds in a loading liquid, taking out the seeds, soaking the seeds in a vitrification reagent, taking out the seeds after soaking is finished, adding a pre-cooled vitrification protection reagent, and transferring the seeds into liquid nitrogen for ultralow-temperature preservation. According to the method, a technical program for ultralow-temperature preservation of flemingia macrophylla germplasm resources is established, the blank in the technical field of ultralow-temperature preservation of flemingia macrophylla seeds is filled up, the preservation time of the seeds can be prolonged through the ultralow-temperature preservation technology, and it is guaranteed that the seeds keep high vitality. 本发明公开了一种大叶千斤拔种子超低温保存方法,包括
format Patent
fullrecord <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN117859737A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN117859737A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN117859737A3</originalsourceid><addsrcrecordid>eNqNzDEKwkAQheE0FqLeYTxAiiASLSUoNlrZhyF5SRZ2d5bZTcTbG9EDWL3me_8ykxvSIC11ojTapGzlmSe4AOU0KigoInTiZMSTdNRZOON7w-S4UQnDy1qmCLSR2LdkXFCZ4ODTh_fQmX_fcxHrbNGxjdj8dpVtL-dHdc0RpEYM3MAj1dW9KMrD_ljuytPuH_MGxYpETQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Method for ultralow-temperature preservation of flemingia macrophylla seeds and improvement of germination rate</title><source>esp@cenet</source><creator>WEI YING ; CHEN XIAOYING ; TAN MEI ; LEI MING ; LIU HAN ; OU XIALIAN ; LI CUI ; ZHANG ZHANJIANG ; HUANG LIRONG ; TANG CHUNFENG ; GUO XIAOYUN ; LI QIAN</creator><creatorcontrib>WEI YING ; CHEN XIAOYING ; TAN MEI ; LEI MING ; LIU HAN ; OU XIALIAN ; LI CUI ; ZHANG ZHANJIANG ; HUANG LIRONG ; TANG CHUNFENG ; GUO XIAOYUN ; LI QIAN</creatorcontrib><description>The invention discloses an ultralow-temperature storage method of moghania macrophylla seeds, which comprises the following steps: 1) under a dark condition, drying the moghania macrophylla seeds by using a silica gel desiccant until the water content is 5.5-9.5%; and 2) soaking the dried seeds in a loading liquid, taking out the seeds, soaking the seeds in a vitrification reagent, taking out the seeds after soaking is finished, adding a pre-cooled vitrification protection reagent, and transferring the seeds into liquid nitrogen for ultralow-temperature preservation. According to the method, a technical program for ultralow-temperature preservation of flemingia macrophylla germplasm resources is established, the blank in the technical field of ultralow-temperature preservation of flemingia macrophylla seeds is filled up, the preservation time of the seeds can be prolonged through the ultralow-temperature preservation technology, and it is guaranteed that the seeds keep high vitality. 本发明公开了一种大叶千斤拔种子超低温保存方法,包括</description><language>chi ; eng</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES ; BLASTING ; DRYING ; DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM ; FERTILISING ; FISHING ; FORESTRY ; HEATING ; HUMAN NECESSITIES ; HUNTING ; LIGHTING ; MECHANICAL ENGINEERING ; PEST REPELLANTS OR ATTRACTANTS ; PLANT GROWTH REGULATORS ; PLANTING ; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF ; SOWING ; TRAPPING ; WEAPONS</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20240412&amp;DB=EPODOC&amp;CC=CN&amp;NR=117859737A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,777,882,25545,76296</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20240412&amp;DB=EPODOC&amp;CC=CN&amp;NR=117859737A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>WEI YING</creatorcontrib><creatorcontrib>CHEN XIAOYING</creatorcontrib><creatorcontrib>TAN MEI</creatorcontrib><creatorcontrib>LEI MING</creatorcontrib><creatorcontrib>LIU HAN</creatorcontrib><creatorcontrib>OU XIALIAN</creatorcontrib><creatorcontrib>LI CUI</creatorcontrib><creatorcontrib>ZHANG ZHANJIANG</creatorcontrib><creatorcontrib>HUANG LIRONG</creatorcontrib><creatorcontrib>TANG CHUNFENG</creatorcontrib><creatorcontrib>GUO XIAOYUN</creatorcontrib><creatorcontrib>LI QIAN</creatorcontrib><title>Method for ultralow-temperature preservation of flemingia macrophylla seeds and improvement of germination rate</title><description>The invention discloses an ultralow-temperature storage method of moghania macrophylla seeds, which comprises the following steps: 1) under a dark condition, drying the moghania macrophylla seeds by using a silica gel desiccant until the water content is 5.5-9.5%; and 2) soaking the dried seeds in a loading liquid, taking out the seeds, soaking the seeds in a vitrification reagent, taking out the seeds after soaking is finished, adding a pre-cooled vitrification protection reagent, and transferring the seeds into liquid nitrogen for ultralow-temperature preservation. According to the method, a technical program for ultralow-temperature preservation of flemingia macrophylla germplasm resources is established, the blank in the technical field of ultralow-temperature preservation of flemingia macrophylla seeds is filled up, the preservation time of the seeds can be prolonged through the ultralow-temperature preservation technology, and it is guaranteed that the seeds keep high vitality. 本发明公开了一种大叶千斤拔种子超低温保存方法,包括</description><subject>AGRICULTURE</subject><subject>ANIMAL HUSBANDRY</subject><subject>BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES</subject><subject>BLASTING</subject><subject>DRYING</subject><subject>DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM</subject><subject>FERTILISING</subject><subject>FISHING</subject><subject>FORESTRY</subject><subject>HEATING</subject><subject>HUMAN NECESSITIES</subject><subject>HUNTING</subject><subject>LIGHTING</subject><subject>MECHANICAL ENGINEERING</subject><subject>PEST REPELLANTS OR ATTRACTANTS</subject><subject>PLANT GROWTH REGULATORS</subject><subject>PLANTING</subject><subject>PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF</subject><subject>SOWING</subject><subject>TRAPPING</subject><subject>WEAPONS</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2024</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNzDEKwkAQheE0FqLeYTxAiiASLSUoNlrZhyF5SRZ2d5bZTcTbG9EDWL3me_8ykxvSIC11ojTapGzlmSe4AOU0KigoInTiZMSTdNRZOON7w-S4UQnDy1qmCLSR2LdkXFCZ4ODTh_fQmX_fcxHrbNGxjdj8dpVtL-dHdc0RpEYM3MAj1dW9KMrD_ljuytPuH_MGxYpETQ</recordid><startdate>20240412</startdate><enddate>20240412</enddate><creator>WEI YING</creator><creator>CHEN XIAOYING</creator><creator>TAN MEI</creator><creator>LEI MING</creator><creator>LIU HAN</creator><creator>OU XIALIAN</creator><creator>LI CUI</creator><creator>ZHANG ZHANJIANG</creator><creator>HUANG LIRONG</creator><creator>TANG CHUNFENG</creator><creator>GUO XIAOYUN</creator><creator>LI QIAN</creator><scope>EVB</scope></search><sort><creationdate>20240412</creationdate><title>Method for ultralow-temperature preservation of flemingia macrophylla seeds and improvement of germination rate</title><author>WEI YING ; CHEN XIAOYING ; TAN MEI ; LEI MING ; LIU HAN ; OU XIALIAN ; LI CUI ; ZHANG ZHANJIANG ; HUANG LIRONG ; TANG CHUNFENG ; GUO XIAOYUN ; LI QIAN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN117859737A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2024</creationdate><topic>AGRICULTURE</topic><topic>ANIMAL HUSBANDRY</topic><topic>BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES</topic><topic>BLASTING</topic><topic>DRYING</topic><topic>DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM</topic><topic>FERTILISING</topic><topic>FISHING</topic><topic>FORESTRY</topic><topic>HEATING</topic><topic>HUMAN NECESSITIES</topic><topic>HUNTING</topic><topic>LIGHTING</topic><topic>MECHANICAL ENGINEERING</topic><topic>PEST REPELLANTS OR ATTRACTANTS</topic><topic>PLANT GROWTH REGULATORS</topic><topic>PLANTING</topic><topic>PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF</topic><topic>SOWING</topic><topic>TRAPPING</topic><topic>WEAPONS</topic><toplevel>online_resources</toplevel><creatorcontrib>WEI YING</creatorcontrib><creatorcontrib>CHEN XIAOYING</creatorcontrib><creatorcontrib>TAN MEI</creatorcontrib><creatorcontrib>LEI MING</creatorcontrib><creatorcontrib>LIU HAN</creatorcontrib><creatorcontrib>OU XIALIAN</creatorcontrib><creatorcontrib>LI CUI</creatorcontrib><creatorcontrib>ZHANG ZHANJIANG</creatorcontrib><creatorcontrib>HUANG LIRONG</creatorcontrib><creatorcontrib>TANG CHUNFENG</creatorcontrib><creatorcontrib>GUO XIAOYUN</creatorcontrib><creatorcontrib>LI QIAN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>WEI YING</au><au>CHEN XIAOYING</au><au>TAN MEI</au><au>LEI MING</au><au>LIU HAN</au><au>OU XIALIAN</au><au>LI CUI</au><au>ZHANG ZHANJIANG</au><au>HUANG LIRONG</au><au>TANG CHUNFENG</au><au>GUO XIAOYUN</au><au>LI QIAN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method for ultralow-temperature preservation of flemingia macrophylla seeds and improvement of germination rate</title><date>2024-04-12</date><risdate>2024</risdate><abstract>The invention discloses an ultralow-temperature storage method of moghania macrophylla seeds, which comprises the following steps: 1) under a dark condition, drying the moghania macrophylla seeds by using a silica gel desiccant until the water content is 5.5-9.5%; and 2) soaking the dried seeds in a loading liquid, taking out the seeds, soaking the seeds in a vitrification reagent, taking out the seeds after soaking is finished, adding a pre-cooled vitrification protection reagent, and transferring the seeds into liquid nitrogen for ultralow-temperature preservation. According to the method, a technical program for ultralow-temperature preservation of flemingia macrophylla germplasm resources is established, the blank in the technical field of ultralow-temperature preservation of flemingia macrophylla seeds is filled up, the preservation time of the seeds can be prolonged through the ultralow-temperature preservation technology, and it is guaranteed that the seeds keep high vitality. 本发明公开了一种大叶千斤拔种子超低温保存方法,包括</abstract><oa>free_for_read</oa></addata></record>
fulltext fulltext_linktorsrc
identifier
ispartof
issn
language chi ; eng
recordid cdi_epo_espacenet_CN117859737A
source esp@cenet
subjects AGRICULTURE
ANIMAL HUSBANDRY
BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES
BLASTING
DRYING
DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
FERTILISING
FISHING
FORESTRY
HEATING
HUMAN NECESSITIES
HUNTING
LIGHTING
MECHANICAL ENGINEERING
PEST REPELLANTS OR ATTRACTANTS
PLANT GROWTH REGULATORS
PLANTING
PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF
SOWING
TRAPPING
WEAPONS
title Method for ultralow-temperature preservation of flemingia macrophylla seeds and improvement of germination rate
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T20%3A14%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=WEI%20YING&rft.date=2024-04-12&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN117859737A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true