Large-view-field high-resolution microscopic imaging method based on partially coherent coded illumination

The invention discloses a large-view-field high-resolution microscopic imaging method based on partially coherent coded illumination. According to the method, a programmable LED array is used as an illumination light source, and four asymmetric illumination patterns and n concentric fan-shaped illum...

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Hauptverfasser: SHU YEFENG, ZUO CHAO, CHEN QIAN, CHEN CHAOJUN, FAN YAO, SUN JIASONG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a large-view-field high-resolution microscopic imaging method based on partially coherent coded illumination. According to the method, a programmable LED array is used as an illumination light source, and four asymmetric illumination patterns and n concentric fan-shaped illumination patterns are utilized to respectively obtain bright and dark field intensity images of an object under a low numerical aperture; complex amplitude of an object is initialized by using a differential phase contrast algorithm, a dark field intensity image is used as intensity constraint, and high-frequency information of the object is recovered through multiple times of iteration updating, so that large-view-field high-resolution microscopic imaging is realized. According to the invention, high-angle partial coherence dark field illumination and bright field illumination are combined, large-view-field images can be recorded under the condition that the spatial resolution is not reduced, and a non-intrusive, h