Method for determining ampicillin residual quantity in recombinant cholera toxin B subunit

The invention belongs to the field of medicine quality detection, and particularly relates to a method for determining ampicillin residual quantity in a recombinant cholera toxin B subunit. Wherein the detection conditions of the high performance liquid chromatography are as follows: a mobile phase: a phase A is a mixed solution of 0.008-0.012 mol/L monopotassium phosphate solution and acetonitrile in a volume ratio of (2-4): 1, and the pH value of the monopotassium phosphate solution is adjusted to 2.95-3.05 by using phosphoric acid; the phase B is acetonitrile; the elution mode is gradient elution, and the elution procedure is 0-8 min, 100% of a mobile phase A and 0% of a mobile phase B; 8-10 min, 80% of the mobile phase A and 20% of the mobile phase B are adopted; in 10-13 minutes, 80% of the mobile phase A and 20% of the mobile phase B are adopted; the mobile phase A accounts for 100%, and the mobile phase B accounts for 0%; 15-25 min, 100% of the mobile phase A and 0% of the mobile phase B are adopted. T