Method for determining content of nitrate and nitrite in nitroglycerin tablet

The invention relates to the technical field of pharmaceutical analysis, and provides a method for determining the content of nitrate and nitrite in a nitroglycerin tablet, which comprises the following steps: extracting the nitroglycerin tablet to obtain a test solution, detecting the test solution by ion pair liquid chromatography, and determining the content of nitrate and nitrite in the nitroglycerin tablet. When the ion pair liquid chromatography is used for detection, a mobile phase A is acetonitrile, and a mobile phase B is formed by mixing an ion pair reagent and a monopotassium phosphate buffer solution. According to the technical scheme, the problems of poor specificity, low sensitivity and low accuracy during detection of nitrate and nitrite in nitroglycerin in the prior art are solved. 本发明涉及药物分析技术领域，提出了一种测定硝酸甘油片中硝酸盐和亚硝酸盐含量的方法，硝酸甘油片经萃取处理后，得到供试品溶液，采用离子对液相色谱法检测供试品溶液，测定硝酸甘油片中硝酸盐和亚硝酸盐的含量；离子对液相色谱法检测时，流动相A为乙腈，流动相B由离子对试剂和磷酸二氢钾缓冲液混合而成。通过上述技术方案，解决了现有技术中的检测硝酸甘油中硝酸盐和亚硝酸盐时，专属性差、灵敏度低和准确度低的问题。