TriFC test system for detecting compound formed by three plant proteins and application thereof

The invention relates to the technical field of verification of interaction of multiple fluorescent molecular proteins and formation of a complex, in particular to a TriFC test system for detecting a complex formed by three plant proteins and application of the TriFC test system. The TriFC test system comprises: 1) an expression cassette A which expresses a first fusion protein by VN154 and a first target gene CDS which are connected in series; 2) an expression cassette B which expresses a second fusion protein by a second target gene CDS sequence and VN156-173 which are connected in series; 3) an expression cassette C which expresses a third fusion protein by a third target gene CDS sequence and VN174 which are connected in series; the sequences of the VN154, the VN156-173 and the VN174 are sequentially as shown in SEQ ID NO. 1 to SEQ ID NO. 3. Compared with a yeast three-hybrid verification protein complex, the TriFC test system provided by the invention can more simply, visually and sensitively detect mult