Extraction method of exosome total RNA

The invention discloses a method for extracting total RNA (Ribonucleic Acid) of an exosome. The method comprises the following steps: step 1, adding QIAzol into a resuspended exosome, uniformly mixing, incubating at room temperature for 4-6 minutes, adding chloroform, uniformly mixing, and incubating at room temperature for 3-5 minutes, wherein the volume ratio of the chloroform to the QIAzol is (0.15-0.25): 1; centrifuging, transferring an upper-layer water phase into a new centrifuge tube, adding alcohol with the volume being 1.4-1.6 times that of the upper-layer water phase, and fully and uniformly mixing; step 2, sucking the uniformly mixed liquid into a centrifugal column, and centrifuging until all the uniformly mixed liquid passes through the centrifugal column; 3, Buffer RWT is added, centrifugation is carried out, RPE is added, the volume ratio of RPE to Buffer RWT is (0.5-1): 1, centrifugation is carried out, and centrifugal waste liquid is discarded; step 4, adding 78-85% alcohol, centrifuging, dis