Rapid PCR (Polymerase Chain Reaction) heat cycle system

The invention provides a rapid PCR (Polymerase Chain Reaction) thermal cycle system. The rapid PCR thermal cycle system comprises a heater, a reaction chip and a radiator, specifically, the heater is located above the reaction chip, and the radiator is located below the reaction chip. Wherein the he...

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Hauptverfasser: ZENG JUNXIANG, JIANG LIMIN, ZHAO XIA, SHEN YUANHENG
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention provides a rapid PCR (Polymerase Chain Reaction) thermal cycle system. The rapid PCR thermal cycle system comprises a heater, a reaction chip and a radiator, specifically, the heater is located above the reaction chip, and the radiator is located below the reaction chip. Wherein the heater is composed of a controller and an LED array, and the controller controls on-off of each LED unit in the LED array so as to realize optical heating of the reaction microdroplets; the reaction chip is of a PMMA-PDMS-PMMA structure, the reaction chip is provided with a reaction cavity, and a single-layer planar microdroplet array can be formed in the cavity; and the reaction chip is modified, so that the thermal conversion efficiency and the nucleic acid amplification reaction effect are improved. 本发明提供了快速PCR热循环系统,包括加热器,反应芯片和散热器;具体地,所述加热器位于所述反应芯片的上方,所述散热器位于所述反应芯片的下方;其中,所述加热器由控制器和LED阵列构成,控制器控制LED阵列中每个LED单元的开关,实现反应微滴的光学加热;所述反应芯片由为PMMA-PDMS-PMMA结构,反应芯片具有反应腔,腔内可以形成单层平面微滴阵列;并对对反应芯片进行修饰,提高热转换效率和核酸扩增反应效果。