Real-time visible light colorimetric PCR (Polymerase Chain Reaction) visual quantitative detection method for adulterated meat

The invention discloses a real-time visible light colorimetric PCR (Polymerase Chain Reaction) visual quantitative detection method for adulterated meat. The method comprises the following steps: extracting DNA (deoxyribonucleic acid) in meat products and carrying out PCR (polymerase chain reaction) amplification; extracting a target double-stranded DNA product subjected to PCR amplification by using a magnetic bead of which the surface is modified with a second connector; resuspending the magnetic beads of which the surfaces are combined with the target double-stranded DNA in a hybridization buffer solution, and carrying out melting treatment on the target double-stranded DNA to obtain a sample solution; sequentially adding a specific detection probe and a colorimetric probe into the sample solution, and hybridizing to obtain a detection solution; 4-nitrophenol and sodium borohydride are added into the detection liquid to form a reaction system, and the adulterated meat in the meat product is detected by obs